Identification and molecular characterisation of CmeB, a Campylobacter jejuni multidrug efflux pump
- 作者：Pumbwe ; Lilian ; Piddock ; Laura J.V.
- 关键词：Efflux ; Campylobacter multidrug efflux B ; Multiple antibiotic resistance ; Campylobacter jejuni
- 刊名：FEMS Microbiology Letters
- 出版年：2002
- 期刊代码：34_03781097
- 类别：imm
- 出版时间：January 10, 2002
- 卷：206
- 期：2
- 页码：185-189
- 文件大小：202 K
摘要
A multidrug efflux pump gene (cmeB) was identified from the published Campylobacter jejuni genome sequence. Secondary structural analysis showed that the gene encoded a protein belonging to the resistance nodulation cell division (RND) family of efflux transporters. The gene was inactivated by insertional mutagenesis. Compared with the wild-type strain (NCTC 11168), the resultant knockout strain (NCTC 11168-cmeB::kanr) displayed increased susceptibility to a range of antibiotics including β-lactams, fluoroquinolones, macrolides, chloramphenicol, tetracycline, ethidium bromide, the dye acridine orange and the detergent sodium dodecyl sulfate. Accumulation of ciprofloxacin was increased in the knockout mutant, but carbonyl cyanide m-chlorophenyl hydrazone, a proton motive force inhibitor, had less effect upon ciprofloxacin accumulation in the knockout mutant compared with NCTC 11168. These data show that the identified gene encodes an RND-type multi-substrate efflux transporter, which contributes to intrinsic resistance to a range of structurally unrelated compounds in C. jejuni. This efflux pump has been named CmeB (for Campylobacter multidrug efflux).