The detection of mating type genes of Tuber melanosporum in productive and non productive soils

详细信息	查看全文 | 推荐本文 |

• 作者：Elisa Zampieri^a ; Roberta Rizzello^a ; Paola Bonfante^a ; ^b ; Antonietta Mello^b ; ^{a.mello@ipp.cnr.it}
• 关键词：Tuber melanosporum ; Mating type genes ; qPCR ; Truffle-ground plantation ; Soil
• 刊名：Applied Soil Ecology
• 出版年：2012
• 期刊代码：14_09291393
• 类别：abs
• 出版时间：June, 2012
• 卷：57
• 期：Complete
• 页码：9-15
• 文件大小：374 K

摘要

Truffles are hypogeous ectomycorrhizal fungi. Of all the different species, Tuber melanosporum is one of the most popular on the truffle market. The aim of this work was to set up a protocol in order to check the fertility of a T. melanosporum ground. The correlation between its abundance in soil, the presence of mating type genes and productivity was investigated. Soil sampling was conducted in a truffle-ground over two periods of the T. melanosporum life cycle, and under two different host species, to verify whether the time and plant species can affect the quantity of mycelium in the soil. An effective quantitative PCR protocol was set up and employed to the investigated truffle-orchard.

We found a statistically significant difference in T. melanosporum abundance between the productive and unproductive soils collected in April. Mating type genes for T. melanosporum were detected under productive and formally productive trees and generally not under unproductive trees even though T. melanosporum was detected. In all the three situations the mating type genes were detected when more than 0.3 ng of T. melanosporum DNA was present. Our results suggest combining these approaches to increase knowledge on the fertility of truffle orchards.