Induction of humoral and cellular immunity against latent HSV-1 infections by DNA immunization in BALB/c mice
- 作者：Amir Ghaemi ; Hoorieh Soleimanjahi ; Taravat Bamdad ; Sara Soudi ; Ehsan Arefeian ; Seyed Mahmood Hashemi ; Massoumeh Ebtekar
- 关键词：DNA vaccine ; Herpes simplex virus type-1 ; Humoral immunity ; Cellular immunity ; Latency
- 刊名：Comparative Immunology ; Microbiology and Infectious Diseases
- 出版年：2007
- 期刊代码：52_01479571
- 类别：bio
- 出版时间：July 2007
- 卷：30
- 期：4
- 页码：197-210
- 文件大小：266 K
摘要
Previously, we have reported that the injection of an expression vector containing Herpes simplex virus (HSV) Glycoprotein D-1 (gD-1) generated a significant antibody response in mice and protected them against HSV lethal challenge. We tested its potential to induce antibody and cell mediated immune responses in latently infected mice. Positive control group (KOS) and HSV gD-1 vaccinated mice demonstrated protection against a lethal ocularly challenge of 105.5 plaque-forming units (pfu)/eye of wild HSV-1 versus negative control groups. For neutralizing antibody titers, delayed-type hypersensitivity (DTH), lymphocyte proliferation responses, clinical evaluation and survival following lethal challenge, no considerable difference was observed between mice vaccinated with DNA plasmid and those vaccinated with KOS.
KOS-vaccinated mice demonstrated the ability to completely prevent latency whereas DNA vaccinated group showed some degree of protection and displayed less latency than negative control groups and had considerably high levels of IFN-γ and strong CTL responses versus negative control groups.
It can be concluded that although immunization with the DNA vaccine is more effective in both protecting mice and induction of immune response, however it could not completely block the latent infection in sensory nerves.