Isolation and phylogenetic analysis of pandemic H1N1/09 influenza virus from swine in Jiangsu province of China
- 作者：Guo Zhao ; Qunping Fan ; Lei Zhong ; Yanfang Li ; Wenbo Liu ; Xiaowen Liu ; Song Gao ; Daxin Peng ; Xiufan Liu ; xfliu@yzu.edu.cn
- 关键词：2009 Pandemic H1N1 influenza A virus ; Swine ; Phylogenetic analysis ; Evolution
- 刊名：Research in Veterinary Science
- 出版年：2012
- 期刊代码：272_00345288
- 类别：med
- 出版时间：August, 2012
- 卷：93
- 期：1
- 页码：125-132
- 文件大小：1058 K
摘要
To investigate whether the 2009 pandemic H1N1 influenza A virus was still being transmitted in swine, a total of 1029 nasal swab samples from healthy swine were collected from January to May 2010 in Jiangsu province of China. Eight H1N1 influenza viruses were isolated and identified, and their full length genomes were sequenced. We found that all eight of the H1N1 viruses shared higher than 98.0%sequence identity with the 2009 pandemic virus A/Jiangsu/1/2009 (JS1). In addition, some of these viruses had D225G (3/8) mutations in the receptor binding sites of the hemagglutinin (HA) protein, indicating enhancement of their binding affinity to the sialic 伪2, 3Gal receptor. In conclusion, the 2009 pandemic H1N1 influenza A virus has retro-infected swine from humans in mainland China, and significant viral evolution is still ongoing in this species.