Kinetics of GADD45伪, TP53 and CASP3 gene expression in the rat lens in聽vivo in response to exposure to double threshold dose of UV-B radiation

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• 作者：Konstantin Galichanin^a ; ^b ; ^{konstantin.galichanin@neuro.uu.se} ; Jessica Svedlund^c ; Per Sö ; derberg^b
• 关键词：lens ; UVR ; stress sensing ; GADD45伪 ; TP53 ; CASP3 ; apoptosis
• 刊名：Experimental Eye Research
• 出版年：2012
• 期刊代码：293_00144835
• 类别：med
• 出版时间：April, 2012
• 卷：97
• 期：1
• 页码：19-23
• 文件大小：294 K

摘要

The purpose of the present study was to investigate the evolution of expression of mRNA message for the genes for the genome stress sensor GADD45伪, the apoptosis initiator TP53 and the apoptosis executor CASP3 in the rat lens in聽vivo in response to exposure to UVR around 300聽nm. Forty six week old female albino Sprague-Dawley rats were unilaterally exposed to double threshold dose for cataract induction, 8聽kJ/m² (8.9聽W/m² for 15聽min), of UVR (位_max聽=聽300聽nm). The animals were sacrificed at 1, 5, 24 and 120聽h following exposure to UVR-B. For each of the GADD45伪, TP53 and CASP3 genes, respectively, mRNA expression in the lenses was measured by quantitative RT-PCR. It was found that expression of mRNA for GADD45伪 transiently increases between 5 and 24聽h after exposure. TP53 is slightly downregulated in exposed lenses at 1 and 5聽h after exposure and thereafter the mRNA expression increases with a constant rate of 9.4鈭?0^鈭?聽rel.聽units/h to a 1.8 fold increase at 120聽h after exposure. Expression of mRNA for CASP3 is downregulated at 1, 5 and 24聽h after in聽vivo exposure and then increases with a constant rate of 4.7鈭?0^鈭?聽rel.聽units/h, upto a 1.3 fold upregulation at 120聽h. Double threshold dose of UVR, for short delay onset of cataract, in聽vivo causes a transient upregulation of the stress sensor GADD45伪, a concurrent downregulation of TP53 and CASP3, followed by a constant upregulation of TP53 that precedes a constant upregulation of CASP3.