Species specific primers for identification of Alternaria solani, in combination with analysis of the F129L substitution associates with loss of sensitivity toward strobilurins

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• 作者：Eva Edin ^{eva.edin@slu.se}
• 关键词：Molecular diagnostics ; PCR ; Species-specific primers ; Fungicide resistance ; Alternaria solani
• 刊名：Crop Protection
• 出版年：2012
• 期刊代码：28_02612194
• 类别：abs
• 出版时间：August, 2012
• 卷：38
• 期：Complete
• 页码：72-73
• 文件大小：95 K

摘要

A species specific forward primer was designed in order to identify the phytopathogenic fungus Alternaria solani, which causes early blight on potato. The reverse primer came from another survey of the American population of A.聽solani. The combination of these two primers can be used both as molecular identification of A.聽solani as well as sending the PCR product for sequencing in search for the F129L substitution associated with loss of sensitivity toward fungicides based on strobilurins. The diagnostic method has been successfully used when identifying the causal agent of lesions on potato leaflets during large field assays.