Small molecule analysis using laser desorption/ionization mass spectrometry on nano-coated silicon with self-assembled monolayers

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• 作者：Ö ; mü ; r Ç ; elikb谋ç ; ak^a ; ¹ ; Gö ; khan Demirel^b ; ² ; Erhan Pi艧kin^c ; ^{piskin@hacettepe.edu.tr} ; Bekir Salih^d ; ^{bekir@hacettepe.edu.tr} ; ^{bekirsal@gmail.com}
• 关键词：LDI ; SAMs ; Matrix-free target ; Desorption/ionization ; Small molecule analysis ; Mass spectrometry
• 刊名：Analytica Chimica Acta
• 出版年：2012
• 期刊代码：2_00032670
• 类别：ch
• 出版时间：4 June, 2012
• 卷：729
• 期：Complete
• 页码：54-61
• 文件大小：1158 K

摘要

Matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) is an emerging technique for the determination of the molecular weight of biomolecules and their non-covalent complexes without fragmentation. One problem with this technique is the use of excess amounts of matrices, which may produce intense fragment ions and/or clusters at low mass ranges between 1 and 800 Da. These fragments lead to interference, especially concerning the signals of small target molecules. Here, a simple, reusable, and quite inexpensive approach was demonstrated to improve the effectiveness of laser desorption/ionization mass spectrometry (LDI-MS) analysis, especially for small molecules, without using matrix molecules. In this study, substrates with controllable morphologies and thicknesses were developed based on the self-assembly of silane molecules on silicon surfaces using N-(3-trimethoxysilylpropyl)diethylenetriamine (TPDA) and octadecyltrichlorosilane (OTS) molecules. Prepared substrates with nano-overlayers were successfully used in the analysis of different types of small target molecules, namely acrivastine, l-histidine, l-valine, l-phenylalanine, l-arginine, l-methionine and angiotensin I. Our substrates exhibited clear peaks almost without fragmentation for all target molecules, suggesting that these surfaces provide a number of important advantages for LDI-MS analysis, such as ease of preparation, costs, reusability, robustness, easy handling and preventing fragmentation.