Further knowledge on barley (Hordeum vulgare L.) leaves O-glycosyl-C-glycosyl flavones by liquid chromatography-UV diode-array detection-electrospray ionisation mass spectrometry
- 作者：F. Ferreres ; P.B. Andrade ; P. Valentã ; o ; A. Gil-Izquierdo
- 关键词：O-Glycosyl-C-glycosyl flavones ; Acylated derivatives ; Mass spectrometry ; Hordeum vulgare
- 刊名：Journal of Chromatography A
- 出版年：2008
- 期刊代码：47_00219673
- 类别：ch
- 出版时间：22 February 2008
- 卷：1182
- 期：1
- 页码：56-64
- 文件大小：632 K
摘要
Thirty-seven flavonoids and a hydroxycynnamic acid have been characterized in barley leaves (Hordeum vulgare L.) by liquid chromatography-UV diode-array coupled to ion trap mass spectrometry with electrospray ionisation interface (negative mode). Their structures have been determined by the study of the ion mass fragmentation which characterizes C-glycosyl flavones and O-glycosyl-C-glycosyl flavones, and differentiates di-O-glycosyl flavones from O-diglycosyl-flavones. The majority of them are described for the first time in barley. Saponarin (isovitexin-7-O-glucoside), lutonarin (isoorientin-7-O-glucoside) and isoscoparin-7-O-glucoside derivatives constitute the major part of the detected compounds. Some acylated derivatives are also described, namely, 7-O-[6-acyl]-glucoside and -7-O-[6-acyl]-glc-4′-glucoside of isovitexin, isoorientin and isoscoparin.