Simultaneous analysis and identification of main bioactive constituents in extract of Zizyphus jujuba var. sapinosa (Zizyphi spinosi semen) by high-performance liquid chromatography–photodiode array detection–electrospray mass spectrometry

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• 作者：Jingjing Liu ; Bo Chen ; Shouzhuo Yao
• 关键词：Zizyphi spinosi semen ; Bioactive constituent ; Triterpene saponin ; Cyclopetide alkaloids ; Flavonoids ; HPLC&#x2013 ; DAD&#x2013 ; ESI-MS
• 刊名：Talanta
• 出版年：2007
• 期刊代码：106_00399140
• 类别：ch
• 出版时间：15 February 2007
• 卷：71
• 期：2
• 页码：668-675
• 文件大小：625 K

摘要

A high-performance liquid chromatography (HPLC) coupled with UV photodiode array detection and electrospray mass spectrometry (HPLC–DAD–ESI-MS) was developed for the simultaneous analysis and identification of main bioactive constituents in extract of zizyphi spinosi semen including three flavonoids, three alkaloids and four triterpene saponins. The separation was achieved on a C₁₈ column with a gradient solvent system of 0.08%aqueous formic acid solution-acetonitrile. The compounds were identified with MS-spectra and authentic standards. The contents of jujuboside A, jujuboside B, spinosin and 6-feruloyl spinosin were quantified by using DAD in different extract of zizyphi spinosi semen.