Effect of 2,4-dinitrophenol under non-oxygenated condition in pons–medulla–spinal cord preparations in newborn rats: Comparison with medulla–spinal cord preparations

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• 作者：Chikako Saiki ; Masaya Makino ; Shigeji Matsumoto
• 关键词：Hypoxia ; 2 ; 4-Dinitrophenol ; Uncoupler ; Pons&#x2013 ; medulla&#x2013 ; spinal cord preparations ; Medulla&#x2013 ; spinal cord preparations ; Respiratory frequency
• 刊名：Neuroscience Letters
• 出版年：2007
• 期刊代码：52_03043940
• 类别：neu
• 出版时间：21 February 2007
• 卷：413
• 期：3
• 页码：255-259
• 文件大小：308 K

摘要

We tested whether depression of respiratory frequency (fR) under non-oxygenated artificial cerebrospinal fluid (aCSF) in pons–medulla–spinal cord (PMS) and medulla–spinal cord (MS) preparations is significantly influenced by the mitochondrial uncoupler 2,4-dinitrophenol (2,4-DNP) in newborn rats.Preparations were obtained from 0- to 4-day-old rats, and fR was monitored at the C4 ventral root in environmental temperature (24 °C). 2,4-DNP was dissolved in aCSF (1, 10 or 30 μM; pH 7.4), and we measured fR in PMS (n = 19) and MS (n = 16), both of which were superfused with aCSF equilibrated with oxygenated (95%O₂–5%CO₂) or non-oxygenated (10%O₂–5%CO₂, balanced with pure N₂) gas.Our results showed that: (1) fR was significantly lower in PMS than MS, (2) fR was significantly decreased under non-oxygenated aCSF in both PMS and MS and (3) fR under non-oxygenated aCSF was significantly increased by 2,4-DNP applications at 10 and 30 μM in PMS but not in MS.Our results suggest that depression in fR under non-oxygenated aCSF in PMS and MS may not be due simply to O₂ limitation, and 2,4-DNP has a stimulant effect on the medullary respiratory rhythm generator (RRG) through pontine RRG regulatory mechanisms under non-oxygenated aCSF.