Broad Anti-Infective Activity of Viracea, An Echinacea-derived Product
- 作者：Tracy L. Hartman ; Karen M. Watson ; Lu Yang ; Meryl J. Squires ; Robert W. Buckheit Jr.
- 关键词：Casomorphin ; Opioid peptide ; Caco-2 ; Peptide transport ; Transepithelial transport
- 刊名：Antiviral Research
- 出版年：2008
- 期刊代码：7_01663542
- 类别：imm
- 出版时间：May 2008
- 卷：78
- 期：2
- 页码：A40
- 文件大小：71 K
摘要
Milk is the source of β-casomorphins – biologically active peptides with opioid activity – which are suspected to play various roles in the human body. The local influence of exogenous opioid peptides on gastrointestinal functions has been widely reported. After passing the gut barrier, β-casomorphins may affect the functions of immunological system, as well as dopaminergic, serotoninergic and GABA-ergic systems in brain, regulate the opioid receptor development and elicit behavioral effects. However, possibilities and mechanisms of the intestinal transport of β-casomorphins in human body in vivo have not been reported so far. In our research, the transepithelial transport of μ-opioid receptor agonists – human β-casomorphin-5 and 7(BCM5, BCM7) and antagonist – lactoferroxin A (LCF A) have been investigated using Caco-2 monolayer. In order to determine the pathway of investigated peptide transport across Caco-2 monolayer, two directions of the transport (apical to basolateral and basolateral to apical) have been studied. All investigated peptides were transported across the human intestinal cell line Caco-2 and the curves of cumulative amount of transported peptides in time were linear in each case. In addition, the hydrolysis of β-casomorphins during 60 min of experiment by dipeptidyl peptidase IV was observed. The data suggest the possibility of transport of opioid peptides derived from food across human intestinal mucosa.