Fertility of Mares Inseminated With Frozen-Thawed Semen Processed by Single Layer Centrifugation Through a Colloid

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• 作者：Katheryn L. Cerny ; BS^a ; ^{katcerny82@uky.edu} ; Sydney Hughes ; BS^a ; Juliana R. Campos ; DVM^b ; Robert J. Coleman ; PhD^c ; Mats H.T. Troedsson ; DVM ; PhD ; ACT^a ; Edward L. Squires ; MS ; PhD ; ACT (Hon)^a
• 关键词：Stallion ; Spermatozoa ; Single Layer Centrifugation ; Cryopreservation
• 刊名：Journal of Equine Veterinary Science
• 出版年：2012
• 期刊代码：173_07370806
• 类别：med
• 出版时间：May, 2012
• 卷：32
• 期：5
• 页码：289-291
• 文件大小：102 K

摘要

The aim of this study was to determine whether there was an increase in pregnancy rates when frozen-thawed stallion semen was processed by single layer centrifugation (SLC) through a colloid before insemination. In addition, changes in semen parameters, including motility, were determined before and after SLC. Twenty light-horse mares (aged 3-16 years) and one Thoroughbred stallion (aged 16 years) having average fertility with fresh and cooled semen (>50%per cycle) and displaying a postthaw motility of >35%were used. Control mares were inseminated using 4- 脳 0.5-mL straws (200 脳 10⁶/mL) of frozen-thawed semen. Treatment mares were inseminated with 4 脳 0.5 mL of frozen-thawed semen after processing by SLC. Pregnancy rates were compared using Fisher exact test, and continuous parameters were evaluated by a Student t test. The pregnancy rates at day 14 were not different for the mares inseminated with control versus SLC-processed semen, despite the difference in sperm number (171 脳 10⁶ 卤 21, 59聽脳 10⁶ 卤 25 progressively motile sperm). After frozen-thawed semen was processed by SLC, the percentage progressively motile sperm improved (P < .05), and SLC processing resulted in a 21.8%recovery of spermatozoa. In summary, centrifugation of frozen-thawed semen through a single layer of colloid increased the percentage of motile spermatozoa, but did not improve pregnancy rates after deep horn insemination.