Wolbachia heat shock protein 60 induces pro-inflammatory cytokines and apoptosis in monocytes in聽vitro

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• 作者：Vijayan Kamalakannan^a ; Sreenivas Kirthika^a ; Kalyanaraman Haripriya^a ; Subash Babu^b ; Rangarajan Badri Narayanan^a ; ^{rbn@annauniv.edu} ; ^{arbeen09@gmail.com}
• 关键词：Wolbachia ; Filariasis ; Cytokines ; Monocytes ; Heat shock proteins ; Apoptosis
• 刊名：Microbes and Infection
• 出版年：2012
• 期刊代码：65_12864579
• 类别：imm
• 出版时间：July, 2012
• 卷：14
• 期：7-8
• 页码：610-618
• 文件大小：1137 K

摘要

Recombinant Wolbachia heat shock protein 60 (rWmhsp60) induces gene expression of pro-inflammatory cytokines IL-1尾, IL-6 and TNF-伪 in human monocytic cell line THP-1. In addition, it inhibits the phagocytic activity and does not alter the nitric oxide production by differentiated THP-1 macrophages, which corroborates with no significant change in inducible nitric oxide synthase gene expression in rWmhsp60 treated THP-1 monocytes. Further, 24聽h stimulation of peripheral blood mononuclear cells from normal individuals by rWmhsp60 reveals that monocytes enter the late apoptotic stage, while lymphocytes do not show apoptosis. Thus these findings suggest that rWmhsp60 may contribute to inflammation mediated monocyte dysfunction in filarial pathogenesis.