Expression of β-1,3-glucanase in flax causes increased resistance to fungi
- 作者：Wró ; bel-Kwiatkowska ; Magdalena ; Lorenc-Kukula ; Katarzyna ; Starzycki ; Michal ; Oszmianski ; Jan ; et. al.
- 关键词：Potato β ; -1 ; 3-glucanase ; Pathogen resistance ; Metabolic profiling ; Linum usitatissimum
- 刊名：Physiological and Molecular Plant Pathology
- 出版年：2004
- 期刊代码：183_08855765
- 类别：bio
- 出版时间：November, 2004
- 卷：65
- 期：5
- 页码：245-256
- 文件大小：1.39 M
摘要
Flax (Linum usitatissimum L.) is an annual plant cultivated in moderate climates. Although the genus Linum contains about 230 species, Linum usitatissimum is the only species which fulfills a commercial function, serving as a fiber donor for the textile industry and the production of linseed oil. The flax breeding is often limited due to the decrease in yield caused by fungi infection. The ectopic expression of a potato β-1,3-glucanase cDNA in flax has improved the plant's defence against pathogen. The resistance of transgenic lines against Fusarium oxysporum and Fusarium culmorum was about threefold higher than for the non-transformed plants. In an in vitro experiment the transgenic plant extract inhibited fungi growth and the inhibition was abolished by extract heating and anti-β-glucanase antibody treatment. By further investigation into the molecular basis of transgenic flax resistance, the metabolic profiling of the plant was performed. The significant decrease in the content of carbohydrate, fatty acids and organic acids and the increase in the level of selected amino acids, polyamines and antioxidant were revealed. What is interesting is that the transgenic plants showed a significant decrease in lignin content, which may lead to the improvement of flax fibre retting.