Characterization of enterotoxigenic E. coli (ETEC), Shiga-toxin producing E. coli (STEC) and necrotoxigenic E. coli (NTEC) isolated from diarrhoeic Mediterranean water buffalo calves (Bubalus bubalis)

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• 作者：G. Borriello^a ; M.G. Lucibelli^a ; E. De Carlo^b ; C. Auriemma^a ; D. Cozza^a ; G. Ascione^a ; F. Scognamiglio^a ; G. Iovane^a ; G. Galiero^a ; ^{giorgio.galiero@cert.izsmportici.it}
• 关键词：Escherichia coli ; Toxins ; Mediterranean water buffalo ; Antimicrobial resistance
• 刊名：Research in Veterinary Science
• 出版年：2012
• 期刊代码：272_00345288
• 类别：med
• 出版时间：August, 2012
• 卷：93
• 期：1
• 页码：18-22
• 文件大小：168 K

摘要

Two hundred and twenty Escherichia coli isolates from 314 Mediterranean water buffalo calves less than 4 weeks old affected by severe diarrhoea with a lethal outcome were characterized for the presence of the virulence factors LT, ST, Stx1, Stx2, haemolysins, intimin, CNF1, CNF2, CDT-I, CDT-II, CDT-III, CDT-IV, and F17-related fimbriae (F17a, F17b, F17c, F17d). The prevalence of ETEC, STEC and NTEC were 1.8%, 6.8%and 20.9%, respectively. The ETEC isolates were all LT-positive and ST-negative. The STEC isolates were all Stx and intimin-positive, with Stx1 (80%) more frequent than Stx2 (27%). The NTEC isolates were all CNF and Hly-positive, with CNF2 (83%) more frequent than CNF1 (22%). Susceptibility assays to 11 antimicrobials displayed high rates of resistance (>30%) to antimicrobials tested. These data show that the most prevalent strains in diarrhoeic water buffalo calves were NTEC, mostly CNF2 and HlyA-positive, with strong associations CNF2/CDT-III and CNF2/F17c.