Differential role of YiGanKang Decoction in IL-1尾 induction of IL-1RI and AP-1 in rat hepatic stellate cell

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• 作者：Yaping Zhang^a ; ^{gracezhangyaping@yahoo.com.cn} ; Xuemei Ai^a ; ^{cooky1126@163.com} ; Junxia Zhang^b ; ^{779322983@qq.com} ; Xixian Yao^c ; ^{yaoxixian@sina.cn}
• 关键词：YiGanKang Decoction ; Interleukin-1尾 ; Type I receptor ; Activator protein 1 ; Hepatic stellate cells
• 刊名：Journal of Ethnopharmacology
• 出版年：2012
• 期刊代码：26_03788741
• 类别：pha
• 出版时间：1 June, 2012
• 卷：141
• 期：2
• 页码：599-602
• 文件大小：235 K

摘要

Background/Aim

YiGanKang, a combination of Chinese herbs, has anti-fibrosis effects in chronic liver diseases. The present study tries to demonstrate differential role of YiGanKang Decoction in interleukin-1尾 (IL-1尾) induction of the type I receptor (IL-1RI) and the activator protein 1 (AP-1) in rat hepatic stellate cell (HSC).

Methods

Flow cytometry was used to detect the IL-1RI expression. Electrophoretic mobility shift assays was used to detect AP-1 activity.

Results

IL-1RI expression after IL-1尾 treatment for 0, 2, 10, 60, and 120 min was 227.08 (13.15), 268.43 (8.93), 442.97 (7.00), 367.66 (14.70), and 261.58 (15.08), respectively. The differences between IL-1RI expression after treatment for 10 and 60 min were significantly higher than the corresponding values in the control (P < 0.01, P < 0.01, respectively); After pretreatment with YiGanKang Decoction, IL-1RI expression induced by IL-1尾 was not decrease obviously; IL-1尾 could activate AP-1 in rat HSCs (P < 0.01). Meanwhile YiGanKang Decoction could inhibit activity of AP-1 induced by IL-1尾 (P < 0.01), and the inhibition rate was 42.71%.

Conclusion

YiGanKang Decoction could not decrease IL-1RI expression, but it could inhibit activity of AP-1 in rat HSCs induced by IL-1尾.