Simultaneous determination of six steroidal saponins and one ecdysone in Asparagus filicinus using high performance liquid chromatography coupled with evaporative light scattering detection

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• 作者：Libo Zhou ; Zhihong Cheng ; Daofeng Chen ; ^{dfchen@shmu.edu.cn} ; ^{chendf@yahoo.cn}
• 关键词：HPLC&ndash ; ELSD ; Asparagus filicinus ; Steroidal saponin ; Ecdysone ; Aspafiliosides ; Filiasparoside A ; 20-Hydroxyecdysone ; Quality evaluation
• 刊名：Acta Pharmaceutica Sinica B
• 出版年：2012
• 期刊代码：pca379_22113835
• 类别：pha
• 出版时间：June, 2012
• 卷：2
• 期：3
• 页码：267-273
• 文件大小：533 K

摘要

A high-performance liquid chromatography coupled with an evaporative light scattering detector (HPLC-ELSD) has been developed to evaluate the quality of Asparagus filicinus through a simultaneous determination of six steroidal saponins and one ecdysone, including aspafiliosides A, B, C, E, G, filiasparoside A and 20-hydroxyecdysone. With a C₁₈ analytical column, the seven analytes were separated efficiently using acetonitrile-water as the mobile phase in a gradient program. The method limits of detection ranged 0.125-0.225 渭g, and the method limits of quantitation ranged 0.408-0.720 渭g, respectively. The intra- and inter-day precisions of the method were evaluated and were all less than 3%. All the recoveries for the spiked analytes ranged 95.16%-100.61%. The proposed method was successfully applied to quantify the seven components in thirteen samples from different localities in China.