Protective immunization against visceral leishmaniasis using Leishmania sterol 24-c-methyltransferase formulated in adjuvant

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• 作者：Yasuyuki Goto ; Lisa Y. Bogatzki ; Sylvie Bertholet ; Rhea N. Coler ; Steven G. Reed
• 关键词：Visceral leishmaniasis ; Sterol 24-c-methyltransferase ; Vaccination
• 刊名：Vaccine
• 出版年：2007
• 期刊代码：89_0264410x
• 类别：imm
• 出版时间：16 October 2007
• 卷：25
• 期：42
• 页码：7450-7458
• 文件大小：788 K

摘要

We present here the identification and characterization of Leishmania sterol 24-c-methyltransferase (SMT), as well as data on protection of mice immunized with this Ag formulated in MPL^®-SE. Serological evaluation revealed that SMT is recognized by VL patients. C57BL/6 mice immunized with this vaccine candidate plus MPL^®-SE showed Ag-specific Th1 immune responses characterized by robust production of IFN-γ upon specific Ag re-exposure in vitro. Upon challenge with L. infantum, mice immunized with SMT plus MPL^®-SE showed significant lower parasite burdens in both spleens and livers compared with non-immunized mice or mice injected with adjuvant alone. The results indicate that SMT/MPL^®-SE can be an effective vaccine candidate for use against VL.