Estimation of the subunit stoichiometry of the membrane-associated daptomycin oligomer by FRET

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• 作者：Jawad K. Muraih ; Michael Palmer ; ^{mpalmer@uwaterloo.ca}
• 关键词：Daptomycin ; Lipopeptide antibiotics ; Oligomer stoichiometry ; Fluorescence resonance energy transfer
• 刊名：Biochimica et Biophysica Acta (BBA)/Biomembranes
• 出版年：2012
• 期刊代码：14_00052736
• 类别：bio
• 出版时间：July, 2012
• 卷：1818
• 期：7
• 页码：1642-1647
• 文件大小：541 K

摘要

Daptomycin is a lipopeptide antibiotic that kills Gram-positive bacteria by depolarizing their cell membranes. This antibacterial action of daptomycin is correlated with the formation of membrane-associated oligomers. We here examine the number of subunits contained in one oligomer using fluorescence resonance energy transfer (FRET). The results suggest that the oligomer contains approximately 6 to 7 subunits, or possibly twice this number if it spans both membrane monolayers.