Correlation between a novel calpastatin biosensor and traditional calpastatin assay techniques
- 作者：C.L. Bratcher ; S.A. Grant ; R.C. Stringer ; C.L. Lorenzen
- 关键词：Biosensor ; Calpastatin ; Fluorescence ; Beef ; Tenderness
- 刊名：Biosensors and Bioelectronics
- 出版年：2008
- 期刊代码：12_09565663
- 类别：ch
- 出版时间：15 May 2008
- 卷：23
- 期：10
- 页码：1429-1434
- 文件大小：308 K
摘要
An optical fiber biosensor to detect calpastatin has been investigated as a preliminary step in developing tenderness detection instrumentation. Longissimus dorsi samples were taken from beef carcasses (n = 21) at 0, 24, 36 and 48 h postmortem. Muscle homogenates were assayed for calpastatin activity using traditional methods and an optical fiber biosensor. Warner–Bratzler shear force was also performed on a steak from each carcass at 14 d postmortem. Results demonstrated that the measurements with highest correlation between traditional calpastatin assays and optical biosensor readings were taken at 48 h postmortem (r = 0.597, P ≤ 0.01), suggesting that this is the best time for use of this biosensor in an on-line grading system. This research further advances the development of a calpastatin biosensor and would be useful in laboratory determination of the presence of biologically active calpastatin concentrations.