Retroperitoneal white adipose tissue mitochondrial function and adiponectin expression in response to ovariectomy and 17尾-estradiol replacement
- 作者：Emilia Amengual-Cladera emi.amengual@uib.es ; Isabel Lladó ; isabel.llado@uib.es ; Magdalena Gianotti ; magdalena.gianotti@uib.es ; Ana M. Proenza ana.proenza@uib.es
- 关键词：White adipose tissue ; Ovariectomy ; E2 replacement ; Mitochondria ; Adiponectin ; Sex
- 刊名：Steroids
- 出版年：2012
- 期刊代码：142_0039128x
- 类别：bio
- 出版时间：May, 2012
- 卷：77
- 期：6
- 页码：659-665
- 文件大小：386 K
摘要
Sexual dimorphism has been previously found both in mitochondrial biogenesis and function and in adiponectin expression of retroperitoneal WAT. However, little is known about the E2 effects on WAT mitochondrial function. Accordingly, the aim of this study was to examine in greater depth the role of estrogens in sexual dimorphism. This was accomplished by studying the effects of ovariectomy and E2 replacement on retroperitoneal WAT mitochondrial function. Fourteen-week-old female and ovariectomized (OVX) female Wistar rats were used in this study. The ovariectomy was performed at 5 weeks of age and at 10 weeks of age OVX rats were divided into two experimental groups: OVX, and OVX treated with 17尾-estradiol (E2) (OVX + E2). Subcutaneous injections of E2 (10 渭g/kg/48 h) were administered to the OVX + E2 rats for 4 weeks previous to the sacrifice whereas OVX rats were treated only with the vehicle. Levels of the main markers for mitochondrial biogenesis and function and those representatives of the antioxidant defense system and insulin sensitivity were determined. Additionally, the mRNA levels of the 伪 and 尾 estrogen receptors and of some adipocyte differentiation markers were studied. Our results indicate that retroperitoneal WAT was able to adapt itself to ovariectomy without any changes in mitochondrial function markers or for the adiponectin levels. However, E2 supplementation led to an unexpected decrease in: TFAM protein levels, in LPL, PPAR纬 and adiponectin gene expression and in the systemic HMW adiponectin levels. This decrease is probably due to the down-regulation of the ER伪 mRNA expression to avoid an over-stimulation by E2.