The isolation of lactic acid bacteria from human colonic biopsies after enrichment on lactose derivatives and rye arabinoxylo-oligosaccharides
- 作者：Kontula ; P. ; Suihko ; M.-L. ; Suortti ; T. ; Tenkanen ; M. ; Mattila-Sandholm ; T. ; von Wright ; A.
- 刊名：Food Microbiology
- 出版年：2000
- 期刊代码：100_07400020
- 类别：imm
- 出版时间：February, 2000
- 卷：17
- 期：1
- 页码：13-22
- 文件大小：235 K
摘要
Lactic acid bacteria (LAB) were isolated from human colon biopsies on LAMVAB by enrichment with different substrates such as lactose derivatives, rye arabinoxylo-oligosaccharides and rye fractions. The selected isolates were tested for their ability to adhere to Caco-2 cells. Only Lactobacillus species were enriched under these conditions. From 161 isolates screened, 28%were identified by ribotyping as Lactobacillus rhamnosus, 29%as L. salivarius, 14%as L. cellobiosus, 13%as L. paracasei and the rest remained unidentified.L. rhamnosus was preferentially enriched by lactulose, L. salivarius by lactobionic acid, L. cellobiosus by lactitol and L. paracasei by arabinoxylo-oligosaccharides. The biopsy-derived strains L. rhamnosus E-97948 andL. paracasei E-97949 have potential for further evaluations in their probiotic and technological properties. Lactulose may have prebiotic effects on colonic LAB by favouring their growth.