Use of a modified alcohol dehydrogenase, ADH1, promoter in construction of diacetyl non-producing brewer's yeast

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• 作者：Onnela ; Maija-Leena ; Suihko ; Maija-Liisa ; Penttilä ; Merja ; Kerä ; nen ; Sirkka
• 关键词：Brewer's yeast ; α ; -Acetolactate decarboxylase ; ADH1 Promoter ; Promoter regulation
• 刊名：Journal of Biotechnology
• 出版年：1996
• 期刊代码：53_01681656
• 类别：imm
• 出版时间：August 20, 1996
• 卷：49
• 期：1-3
• 页码：101-109
• 文件大小：840 K

摘要

The bacterial gene, encoding α-acetolactate decarboxylase (α-ALDC), was expressed in a bottom-fermenting brewer's yeast under the control of a modified Saccharomyces cerevisiae alcohol dehydrogenase (ADH1) promoter which lacks the upstream regions from −800 bp to −1500 bp. In pilot scale brewing conditions, the level of α-ALDC active brewer's yeast strains were also shown to be suitable for high gravity brewing.