Glycosyltransferase and its application to glycodiversification of natural products

详细信息	查看全文 | 推荐本文 |

• 作者：Sung Hee Choi^a ; Hee Sook Kim^b ; Yeo Joon Yoon^c ; Dong-Myung Kim^d ; Eun Yeol Lee^a ; ^{eunylee@khu.ac.kr}
• 关键词：Glycosylation ; Glycosyltransferase ; Secondary metabolites ; Glycosyltransferase-A ; Glycosyltransferase-B
• 刊名：Journal of Industrial and Engineering Chemistry
• 出版年：2012
• 期刊代码：P29_1226086x
• 类别：ce
• 出版时间：25 July, 2012
• 卷：18
• 期：4
• 页码：1208-1212
• 文件大小：631 K

摘要

Glycosyltransferase (GT) catalyzes the transfer of a sugar moiety to acceptor substrates such as secondary metabolites. The majority of GTs has two structural folds, GT-A and GT-B based on 3-D structural analysis. The limited structural fold diversity is compensated by a highly divergent acceptor binding domain for conferring sufficient substrate promiscuity. Various GTs have been engineered to further enhance the glucose transfer activity and expand substrate promiscuity by error-prone PCR and site-directed mutagenesis. Engineered GT-catalyzed glycosylation will certainly play a key role in the generation of scaffold for new drug discovery and control of drug pharmacokinetics.