Studies on GM-CSF DNA as an adjuvant for neutralizing Ab elicited by a DNA/MVA immunodeficiency virus vaccine
- 作者：Harriet L. Robinson ; David C. Montefiori ; Francois Villinger ; James E. Robinson ; Sunita Sharma ; Linda S. Wyatt ; Patricia L. Earl ; Harold M. McClure ; Bernard Moss and Rama Rao Amara
- 关键词：Immunodeficiency virus vaccine ; DNA/MVA vaccine ; GM-CSF DNA adjuvant ; Neutralizing Ab ; Avidity of Ab ; Re-emergent virus protection
- 刊名：Virology
- 出版年：2006
- 期刊代码：108_00426822
- 类别：imm
- 出版时间：1 September 2006
- 卷：352
- 期：2
- 页码：285-294
- 文件大小：576 K
摘要
Here, we use a vaccine consisting of DNA priming followed by MVA boosting in rhesus macaques to investigate the ability of GM-CSF DNA to serve as an adjuvant for the elicitation of neutralizing Ab against an HIV-1 Env. The trial used Gag, Pol, and Env sequences from SHIV-89.6 in the immunogens and a neutralization escape variant of SHIV-89.6, SHIV-89.6P, for challenge. Co-delivery of GM-CSF and vaccine DNAs enhanced the temporal appearance of neutralizing Ab and broadened the specificity of the neutralizing activity to include SHIV-89.6P. Two long-term SHIV-89.6 infections elicited neutralizing activity for SHIV-89.6 but not SHIV-89.6P. Studies on the avidity of the anti-Env antisera revealed that the GM-CSF-adjuvanted vaccine had elicited higher avidity Ab than the non-adjuvanted vaccine or the infection. The GM-CSF-adjuvanted group showed a trend towards better control of the challenge infection and had better control of re-emergent virus (P < 0.01) than the non-adjuvanted group.