Use of supercritical carbon dioxide for proteins and alcohol dehydrogenase release from yeast Saccharomyces cerevisiae

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• 作者：M. 膶olnik ; M. Primo啪i膷 ; 沤. Knez ; M. Habulin ; ^{maja.habulin@uni-mb.si}
• 关键词：S. cerevisiae ; Saccharomyces cerevisiae ; SC CO2 ; supercritical carbon dioxide ; ADH ; alcohol dehydrogenase ; NA ; nucleic acid ; NAD+ ; nicotinamide adenine dinucleotide ; CO2 ; carbon dioxide ; (HCO3)&minus ; bicarbonate ion ; PDB ; potato dextrose broth ; SPB ; sod
• 刊名：The Journal of Supercritical Fluids
• 出版年：2012
• 期刊代码：60_08968446
• 类别：ce
• 出版时间：May, 2012
• 卷：65
• 期：Complete
• 页码：11-17
• 文件大小：777 K

摘要

In this study, supercritical carbon dioxide (SC CO₂) was used to obtain proteins and enzymes from yeast Saccharomyces cerevisiae.

The changes in viability and cell morphology, the release of the cellular proteins and the activity alteration of the enzyme alcohol dehydrogenase (ADH) from S. cerevisiae resulting from the exposure to SC CO₂ were investigated. Before the treatment of S. cerevisiae in SC CO₂, the number of viable cells was cca. 10⁵ colony forming units per mL of cell suspension (cfu/mL). The suspension of the S. cerevisiae culture was incubated in SC CO₂ at different pressures (7.5, 15 and 30 MPa) for different treatment times (30-300 min) and at constant temperature (35 掳C). The influences of these parameters on total protein concentration, ADH activity and changes in absorbance of nucleic acids (NA) in the suspension of S. cerevisiae during SC CO₂ treatment were studied. A decrease in number of living cells of S. cerevisiae in potato dextrose broth (PDB) or in sodium pyrophosphate buffer (SPB) exposed to SC CO₂ at all studied pressures was determined when the treatment time was increased. The highest activity of ADH after treatment of the yeast culture suspended in PDB in SC CO₂ was detected at a pressure of 7.5 MPa and at the treatment time of 120 min.The use of SC CO₂ is a suitable option to achieve cell death and consequently the secretion of proteins and ADH from cells of S. cerevisiae.