N,N,N-Trimethyl chitosan nanoparticles for controlled intranasal delivery of HBV surface antigen

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• 作者：Ramesh Subbiah^a ; ^b ; ¹ ; Prakash Ramalingam^c ; ¹ ; Subramaniyan Ramasundaram^b ; Do Yang Kim^b ; Kwideok Park^b ; Mohan K. Ramasamy^c ; ^{thambiavn@gmail.com} ; Kyoung Jin Choi^d ; ^{choi@unist.ac.kr}
• 关键词：Trimethyl chitosan ; Nanoparticles ; Controlled intranasal delivery ; Atomic force microscopy ; In vivo immunological study
• 刊名：Carbohydrate Polymers
• 出版年：2012
• 期刊代码：14_01448617
• 类别：ch
• 出版时间：1 August, 2012
• 卷：89
• 期：4
• 页码：1289-1297
• 文件大小：1498 K

摘要

Hepatitis B virus surface antigen (HBsAg) loaded N,N,N-trimethyl chitosan nanoparticles (N-TMC NPs) were formulated and studied for controlled intranasal delivery. The size and surface properties of the NPs can be tuned by modifying the concentration of N-TMC and found to be 66 卤 13, 76 卤 9 nm for 0.25 and 0.5 wt.%respectively. Loading of 380 and 760 渭l of HBsAg yielded 143 卤 33, 259 卤 47 nm sized spherical N-TMC NPs with highest loading efficiency and capacity of 90-93%, and 96-97%respectively. In vitro drug release analysis ensured 93%cumulative release of HBsAg antigen over prolonged period (43 days). In vivo immunological study was performed using 6-8 weeks old female BALB mice and reveals adjuvants efficiency of NPs for antigen is highly stable and better than standard. Obtained results show that N-TMC NPs can be extensively used in controlled intra nasal delivery to treat various diseases including hepatitis B and allergic rhinitis.