Synthesis of gold nanoparticles bearing the Thomsen脙脙脙脙脙脙Friedenreich disaccharide: a new multivalent presentation of an important tumor antigen
摘要
Herein we describe the synthesis gold nanoshells encapsulated with up to 90 units of the Thomsen芒芒芒Friedenreich (TF) tumor-associated carbohydrate antigen (TACA) disaccharide (Gal芒芒1-3GalNAc-芒芒-O-Ser/Thr) as well as the assembly of a suitably linked designer glycopeptide as a precursor to similar multivalent presentations on gold. The TF-coated nanoparticles are highly stable, water soluble, and easily handled. Improvements in the linker technology used to attach the disaccharide to the particles led to a robust multivalent platform for the presentation of this important carbohydrate. The antigen retains all recognition characteristics while displayed on this template as shown by several in vitro assays. This area of research could lead to the development of novel therapeutic agents that inhibit protein芒芒芒carbohydrate interactions.