Cytotoxicity of selected magnetic fluids on human adenocarcinoma cells
- 作者：Hilger ; Ingrid ; Frü ; hauf ; Sylvia ; Linß ; Werner ; Hiergeist ; Robert ; Andrä ; Wilfried ; et. al.
- 关键词：Magnetic fluids ; Cytotoxicity ; Magnetic thermoablation ; Breast cancer ; Cancer therapy ; Heating
- 刊名：Journal of Magnetism and Magnetic Materials
- 出版年：2003
- 期刊代码：31_03048853
- 类别：ph
- 出版时间：April, 2003
- 卷：261
- 期：1-2
- 页码：7-12
- 文件大小：316 K
摘要
Based on the knowledge that the magnetite particles seem to be well tolerated by the human body, the cytotoxic potential of coated particles was investigated, which had been selected for potential applications regarding the minimal-invasive elimination of breast tumors by magnetic thermoablation. Human adenocarcinoma cells (BT-20) were exposed (24, 48 and 72h) to different magnetite particles with diverging total size (8, 10 and 220nm) and coating (cationic and anionic). One sample contained only non-coated magnetite particles. The magnetite concentration ranged between 0.2 and 20ng/cell. Cytotoxicity was estimated by measuring the succinate dehydrogenase activity. The morphologic features resulting from the interaction of magnetic fluids with BT-20 cells was determined by transmission electron microscopy. As opposed to the non-coated magnetic particles, cationic particles induced the strongest decrease in cell survival rates depending on time and concentration. Morphologically, the cationic particle samples exerted a strong binding to cellular membranes. Changes in the subcellular structure were found in relation to the coated magnetic particles. In conclusion, our results show that the coated prototype magnetic particles, particularly those with a cationic surfactant, are cytotoxic to BT-20 cells. The cytotoxicity is attributed to electrostatic bindings with cellular membranes, influences of chemical components or non-physiologic pH. Considering the in vivo applications, adverse systemic effects are conceivable and more biocompatible coatings for the selected magnetic particles should be elaborated.