Isolation and characterization of na茂ve follicular dendritic cells

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• 作者：Kenta Usui^a ; Shin-ichiro Honda^a ; ^b ; ^{shonda@md.tsukuba.ac.jp} ; Yuichi Yoshizawa^a ; Chigusa Nakahashi-Oda^a ; ^b ; Satoko Tahara-Hanaoka^a ; ^b ; Kazuko Shibuya^a ; Akira Shibuya^a ; ^b
• 关键词：FDCs (follicular dendritic cells) ; BAFF (B cell activating factor TNF family)
• 刊名：Molecular Immunology
• 出版年：2012
• 期刊代码：112_01615890
• 类别：bio
• 出版时间：March, 2012
• 卷：50
• 期：3
• 页码：172-176
• 文件大小：889 K

摘要

Follicular dendritic cells (FDC) are specialized antigen-presenting cells to cognate B cells in the follicle of the lymphoid tissues. FDC also support survival and proliferation of the B cells, leading to the germinal center formation. FDC therefore play a central role in humoral immune responses. However, molecular and functional characteristics of FDC are largely unknown, because it is difficult to isolate and analyze FDC due to a very small number of FDC in the lymphoid tissues and the fragility by mechanical and chemical stresses in vitro. In this report, we established a novel method for FDC isolation from the spleen of na茂ve mice by flow cytometry and analyzed the phenotypical and functional characteristics. The isolated FDC, which accounted for 鈭?.2%of the spleen cells of na茂ve mice, were CD45^{鈭?/sup>, FDC-M2+, and ICAM-1+, and supported the survival and LPS-induced proliferation of B cells. We also showed that a neutralizing antibody against B cell activating factor TNF family (BAFF) suppressed FDC-dependent B cell proliferation in the presence of LPS, but not survival, demonstrating the evidence that FDC-derived BAFF is involved in B cell proliferation.}