Synthesis of a biotinylated camptothecin derivative and determination of the binding sequence by T7 phage display technology

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• 作者：Yoichi Takakusagi ; Keisuke Ohta ; Kouji Kuramochi ; Kengo Morohashi ; Susumu Kobayashi ; Kengo Sakaguchi and Fumio Sugawara
• 关键词：Camptothecin ; Phage display ; Biotin
• 刊名：Bioorganic and Medicinal Chemistry Letters
• 出版年：2005
• 期刊代码：10_0960894x
• 类别：ch
• 出版时间：2005
• 卷：15
• 期：21
• 页码：4846-4849
• 文件大小：155 K

摘要

A biotinylated derivative of the anti-tumor agent camptothecin (CPT) was synthesized and used in a phage display assay to identify drug-binding sequences. After three rounds of selection using C20-biotinylated CPT (CPT-20-B) as bait, a CPT-20-B-binding sequence, NSSQSARR, was identified.