The aim of our experiments was to identify new binding sites for intermediate filaments on plectin and to specify these sites.
We introduced truncated forms of plectin into several cell lines and observe interaction between plectin and intermediate filaments.
We found that a linker region in the COOH-terminal end of plectin was required for the association of the protein with intermediate filaments. In addition, we also demonstrated that a serine residue at position 4645 of plectin may have a role on binding of plectin to intermediate filaments.
A linker region in the COOH-terminal end and serine residue at position 4645 may be important for the binding of plectin to intermediate filaments.