Characterization of immune modulating functions of 纬未 T cell subsets in a gnotobiotic pig model of human rotavirus infection
- 作者：Ke Wen ; Tammy Bui ; Guohua Li ; Fangning Liu ; Yanru Li ; Jacob Kocher ; Lijuan Yuan ; lyuan@vt.edu
- 关键词：Gammadelta (纬未) T cell subset ; Rotavirus ; Gnotobiotic pigs ; Toll-like receptors ; Cytokines ; T cell proliferation
- 刊名：Comparative Immunology ; Microbiology and Infectious Diseases
- 出版年：2012
- 期刊代码：52_01479571
- 类别：bio
- 出版时间：July, 2012
- 卷：35
- 期：4
- 页码：289-301
- 文件大小：1501 K
摘要
We characterized immune modulating functions of porcine 纬未 T cell subsets in rotavirus infection using a gnotobiotic pig model of human rotavirus infection and sort-purified lymphocyte autologous co-cultures. We demonstrated that CD2+CD8鈭?and CD2鈭扖D8鈭?纬未 T cells have mainly pro-inflammatory function as evident by directly secreting IFN-纬 or promoting CD4+ 伪尾 T cell proliferation and IFN-纬 production, whereas CD2+CD8+ 纬未 T cells mainly exert regulatory T cell function by expressing FoxP3, secreting IL-10 and TGF-尾 or increasing IL-10 and TGF-尾 production by CD4+ 伪尾 T cells. 纬未 T cells responded to rotavirus infection by increasing TLR2, TLR3, TLR9 expression and IFN-纬 and/or TGF-尾 production. The CD8鈭?subsets likely differentiate into CD8+ subset by acquiring CD8 expression, explaining in part the apparently dual functions of CD2+CD8+ and CD2+CD8鈭?subsets. Thus, both CD8+ and CD8鈭?纬未 T cell subsets can contribute to anti-rotavirus immunity and to the maintenance and restoration of intestinal and systemic homeostasis.