Reference intervals of bone turnover markers in healthy premenopausal women: Results from a cross-sectional European study

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• 作者：Richard Eastell^a ; ^{r.eastell@sheffield.ac.uk} ; Patrick Garnero^b ; Christine Audebert^c ; David L. Cahall^d
• 关键词：Assays ; Bone turnover markers ; Osteoporosis ; Premenopausal women ; Reference intervals
• 刊名：Bone
• 出版年：2012
• 期刊代码：39_87563282
• 类别：med
• 出版时间：May, 2012
• 卷：50
• 期：5
• 页码：1141-1147
• 文件大小：322 K

摘要

Robust validated reference intervals for bone turnover markers (BTMs) are required to assess fracture risk and effectiveness of therapy. However, there are currently limited reference intervals for BTMs in premenopausal women, especially comparing manual and automated assays. This study determined the BTM reference intervals using automated and manual assays, compared the results obtained from two different assays, and evaluated the factors that may affect BTM levels. This was a cross-sectional registry study in 194 healthy, premenopausal, European Caucasian women aged 35 to 39 years from France (n = 98) and Denmark (n = 96). Two independent specialized laboratories, one in France (Synarc) and the other in Denmark (Nordic Bioscience), analyzed blood and urine samples from each woman for BTM levels.

The type of assay used in this study significantly affected the reference intervals obtained for serum cross-linked C-terminal telopeptide of type I collagen (sCTX) and urinary cross-linked N-terminal telopeptides of type I collagen (uNTX/Cr; both P < 0.001), but not for serum procollagen type I amino-terminal propeptide (PINP; P = 0.28). The Serum Crosslaps庐 ELISA; Microtitre-plate based ELISA; Metra BAP EIA; and UniQ庐 PINP RIA assays yielded higher BTM reference values. The reference intervals for the BTMs, as measured with Serum 尾-Crosslaps, Elecsys庐 2010 Systems; VITROS庐 ECI System; Ostase庐, Access庐 Immunoassay System; and Total PINP, Elecsys庐 2010 Systems assays, were 0.111-0.791 ng/mL for sCTX, 12.3-59.7 nmol BCE/mmol creatinine for uNTX/Cr, 5.8-17.5 ng/mL for bone alkaline phosphatase (ALP), and 17.3-83.4 ng/mL for PINP, respectively.

When measured with Serum Crosslaps庐 ELISA, Microtitre-plate based ELISA, Metra BAP EIA, and UniQ庐 PINP RIA, the reference intervals were 0.177-0.862 ng/mL for sCTX, 22.6-95.7 nmol BCE/mmol creatinine for uNTX/Cr, 14.8-38.8 U/L for bone ALP, and 19.5-75.2 ng/mL for PINP, respectively. The clinical interpretation of the BTMs of a subject should be based on comparison with a BTM reference interval, measured with the same assay method.