Spatio-temporal Expression Study of Phosphorylated 70-kDa Ribosomal S6 Kinase (p70S6k) in Mesial Temporal Lobe Epilepsy

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• 作者：Xiao-liang Xing^a ; ^&dagger ; ; Long-ze Sha^a ; Yuan Yao^b ; Yan Shen^a ; Li-wen Wu^b ; ^{wlw1946@yahoo.com.cn} ; Qi Xu^a ; ^{qixu@vip.sina.com}
• 关键词：mesial temporal lobe epilepsy ; p70S6k ; kainate acid ; immunoreactivity
• 刊名：Chinese Medical Sciences Journal
• 出版年：2012
• 期刊代码：P74_10019294
• 类别：med
• 出版时间：March, 2012
• 卷：27
• 期：1
• 页码：7-10
• 文件大小：911 K

摘要

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Objective

To determine the spatio-temporal expression of p70S6k activation in hippocampus in mesial temporal lobe epilepsy.

Methods

Temporal lobe epilepsy model was established by stereotaxically unilateral and intrahip-pocampal injection of kainite acid (KA) in adult male C57BL/6 mice. Latent and chronic epileptogenesis were represented by mice 5 days after KA injection (n=5) and mice 5 weeks after KA injection (n=8), respectively. Control mice (n=5) were injected with saline. Immunohistochemical assays were performed on brain sections of the mice.

Results

Hippocampus both ipsilateral and contralateral to the KA injection displayed significantly up-regulated pS6 immunoreactivity in dispersed granule cells in 5-day and 5-week model mice.

Conclusion

The activation of p70S6k is mainly located in the dentate gyrus in KA-induced mouse model of temporal lobe epilepsy, indicating that the activation may be related with the disperse degree and hypertrophy of granule cells.