Protein adsorption at liquid/liquid interface with low interfacial tension
- 作者：Kiss ; É ; va ; Borbá ; s ; Ré ; ka
- 关键词：Protein adsorption ; Dilatational rheology ; Emulsion stability ; Three-phase partitioning
- 刊名：Colloids and Surfaces B ; Biointerfaces
- 出版年：2003
- 期刊代码：14_09277765
- 类别：ms
- 出版时间：September 1, 2003
- 卷：31
- 期：1-4
- 页码：169-176
- 文件大小：329 K
摘要
Adsorption of proteins at the interface of two-liquid systems composed of aqueous ammonium sulfate solution and tert-butanol by phase separation was investigated by drop shape tensiometry. The change of interfacial tension with time and protein concentration as well as upon compression of the adsorbed layer were compared for bovine serum albumin, ovalbumin, β-lactoglobulin, lysozyme, trypsin and horse radish peroxidase. A correlation between the dilatational moduli of various protein films and the partitioning of proteins in the two-liquid system was found, which provides evidence to the role of emulsion stability in protein separation by three-phase partitioning.