• 作者：S. Lef&#232 ; vre ; ^{sophie.lefevre@chru-strasbourg.fr} ; V. Cocquerelle ; P. Riegel ; J.-M. Scheftel ; B. Jaulhac ; F. Jehl
• 关键词：Vitek-2&trade ; AIX ; Vitek-2&trade ; PC ; Enté ; robacté ; ries ; 尾-lactamines ; Antibiogramme
• 刊名：Pathologie Biologie
• 出版年：2012
• 期刊代码：216_03698114
• 类别：med
• 出版时间：April, 2012
• 卷：60
• 期：2
• 页码：78-83
• 文件大小：429 K

Aim of the study

Vitek-2鈩?AIX versus Vitek-2鈩?PC have different rules for phenotypic interpretation. The aim of this study is to ensure that the raw results determined by these two versions of Vitek-2鈩?allow biologists to conclude to the same resistance phenotype, but also to evaluate their own phenotypic interpretation system (advanced expert system).

Material and methods

A total of 251聽strains of Enterobacteriaceae of different groups and phenotypes was tested. Each strain was studied simultaneously on both types of Vitek-2鈩?from the same calibrated inoculum. We then compared their resistance phenotype to beta-lactams.

Results

For strains not producing ESBL or CHN, the biologist concluded in 99.3%of cases to the same resistance phenotype by interpreting the raw results of Vitek-2鈩?AIX versus PC. The phenotypic interpretation of biologist is different from the Vitek-2鈩?in respectively 40%versus 43%of cases for AIX and PC versions. For multi-resistant strains, the biologist concluded in 100%of cases to the same resistance phenotype by interpreting the raw results of Vitek-2鈩?AIX versus PC. In 51.5%of cases the biologist use the disk diffusion method (DD). The results of this technique put forward 29%discrepancy with the two types of Vitek-2鈩? Finally, when Vitek-2鈩?claims the presence of an ESBL alone, this result is routinely confirmed by DD.

Conclusion

The switch fromVitek-2鈩?AIX to Vitek-2鈩?PC does not alter the results of the phenotypic interpretation of biologist.