Platelet activating factor induces cytoskeletal reorganization through Rho family pathway in THP-1 macrophages
- 作者：Chinuyo Sumita ; Masako Yamane ; Takahisa Matsuda ; Makiko Maeda ; Tetsuro Nariai ; Yasushi Fujio and Junichi Azuma
- 关键词：PAF ; Rho family proteins ; Macrophage ; Cytoskeletal reorganization
- 刊名：FEBS Letters
- 出版年：2005
- 期刊代码：70_00145793
- 类别：bio
- 出版时间：2005
- 卷：579
- 期：18
- 页码：4038-4042
- 文件大小：252 K
摘要
In the process of atherosclerosis, platelet activating factor (PAF) promotes the infiltration of inflammatory cells into atherosclerotic plaque by modulating their cytoskeleton. Here, we examined whether Rho family proteins are involved in PAF-induced cytoskeletal reorganization in THP-1 macrophages. PAF stimulation rapidly induced cell elongation, accompanied by filopodia formation. The inhibition of Rho family proteins by the overexpression of Rho-GDI attenuated the PAF-mediated morphological changes. Both RhoA and Cdc42 were activated in response to PAF. Inhibition of RhoA or Cdc42 by dominant negative mutants abrogated morphological changes induced by PAF. Collectively, PAF regulates cytoarchitecture through Rho family proteins in macrophages.