On-line stacking techniques for the nonaqueous capillary electrophoretic determination of acrylamide in processed food

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• 作者：Filiz Tezcan ; F. Bedia Erim
• 关键词：Acrylamide ; Nonaqueous capillary electrophoresis ; Sample stacking ; Food
• 刊名：Analytica Chimica Acta
• 出版年：2008
• 期刊代码：2_00032670
• 类别：ch
• 出版时间：9 June 2008
• 卷：617
• 期：1-2
• 页码：196-199
• 文件大小：231 K

摘要

In the present study, field amplified sample stacking (FASS) techniques in the nonaqueous capillary electrophoresis method (NACE) were introduced for the on-line concentration of the acrylamide to improve acrylamide detection at 210 nm by diode-array detection. Acetonitrile (ACN) as a nonaqueous solvent permits acrylamide to be protonated through the change of its acid–base chemistry, allowing capillary electrophoretic separation of this compound. Choosing 30 mmol L⁻¹ HClO₄, 20 mmol L⁻¹ NaClO₄, 218 mmol L⁻¹ CH₃COOH in ACN as the separation electrolyte and employing sample stacking methods, the LOD value of acrylamide was decreased to 2.6 ng mL⁻¹ with electrokinetic injection and 4.4 ng mL⁻¹ with hydrodynamic injection. Optimized stacking conditions were applied to the determination of acrylamide in several foodstuffs. The method is simple, rapid, inexpensive, and widely applicable for the determination of acrylamide in food samples.