Estrogen attenuates hypoxic脙脙脙脙脙脙ischemic brain injury in neonatal rats
- 作者：Feng ; Yangzheng ; Fratkins ; Jonathan D. ; LeBlanc ; Michael H.
- 关键词：Stroke ; Neuroprotection ; Apoptosis ; Thiobarbituric acid reacting substance ; Oxygen radical
- 刊名：European Journal of Pharmacology
- 出版年：2005
- 期刊代码：24_00142999
- 类别：neu
- 出版时间：January 10, 2005
- 卷：507
- 期：1-3
- 页码：77-86
- 文件大小：330 K
摘要
Estrogen is neuroprotective in adult animals. We wished to determine if estrogen protects against brain injury in the newborn. Four-day-old rat pups were treated with subcutaneously implanted pellets containing 0.05 mg (2.4 μg/day) of 17β-estradiol or vehicle, designed to release the estrogen over 21 days. At 7 days old the pups had the right carotid artery ligated followed by 2.5 h of 8%oxygen. Brain damage was evaluated by weight deficit of the right hemisphere at 22 days following hypoxia. Estradiol treatments reduced brain weight loss from −17.4±2.8%S.E.M. in the vehicle group (n=32) to −9.3±2.7%in the treated group (n=32, P<0.05). Brain cortex thiobarbituric acid reacting substances and caspase activities were assessed 24 h after reoxygenation. Estradiol significantly reduced a hypoxia-induced increase in brain thiobarbituric acid reactive substances (P<0.05). Levels of caspase-3, -8 and -9 activity increased due to hypoxia–ischemia. Estradiol had no effect on caspase activity. Estradiol reduced brain injury in the neonatal rat.