In聽Vivo Imaging of Tumor-Propagating Cells, Regional Tumor Heterogeneity, and Dynamic Cell Movements in Embryonal Rhabdomyosarcoma

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• 作者：Myron&#160 ; S. Ignatius¹ ; ³ ; Eleanor Chen¹ ; ³ ; ⁴ ; Natalie&#160 ; M. Elpek² ; Adam Z. Fuller¹ ; ³ ; Inê ; s&#160 ; M. Tenente¹ ; ³ ; ⁵ ; Ryan Clagg¹ ; ³ ; Sali Liu¹ ; ³ ; Jessica&#160 ; S. Blackburn¹ ; ³ ; Corinne&#160 ; M. Linardic⁶ ; Andrew E. Rosenberg¹ ; Petur&#160 ; G. Nielsen¹ ; Thorsten&#160 ; R. Mempel² ; David&#160 ; M. Langenau¹ ; ³ ; ^{dlangenau@partners.org}
• 刊名：Cancer Cell
• 出版年：2012
• 期刊代码：43_15356108
• 类别：med
• 出版时间：15 May, 2012
• 卷：21
• 期：5
• 页码：680-693
• 文件大小：3481 K

摘要

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Summary

Embryonal rhabdomyosarcoma (ERMS) is an aggressive pediatric sarcoma of muscle. Here, we show that ERMS-propagating potential is confined to myf5+ cells and can be visualized in live, fluorescent transgenic zebrafish. During early tumor growth, myf5+ ERMS cells reside adjacent normal muscle fibers. By late-stage ERMS, myf5+ cells are reorganized into distinct regions separated from differentiated tumor cells. Time-lapse imaging of late-stage ERMS revealed that myf5+ cells populate newly formed tumor only after seeding by highly migratory myogenin+ ERMS cells. Moreover, myogenin+ ERMS cells can enter the vasculature, whereas myf5+ ERMS-propagating cells do not. Our data suggest that non-tumor-propagating cells likely have important supportive roles in cancer progression and facilitate metastasis.