Analysis of pork adulteration in commercial meatballs targeting porcine-specific mitochondrial cytochrome b gene by TaqMan probe real-time polymerase chain reaction

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• 作者：M.E. Ali^a ; ^{eaqubali@gmail.com} ; U. Hashim^b ; S. Mustafa^c ; Y.B. Che Man^c ; ; Th.S. Dhahi^b ; M. Kashif^b ; Md. Kamal Uddin^d ; S.B. Abd Hamid^a
• 关键词：Meatball formulation ; Double quenched TaqMan probe ; Halal and Kosher foods ; Threshold cycle ; Ground meat
• 刊名：Meat Science
• 出版年：2012
• 期刊代码：78_03091740
• 类别：abs
• 出版时间：August, 2012
• 卷：91
• 期：4
• 页码：454-459
• 文件大小：601 K

摘要

A test for assessing pork adulteration in meatballs, using TaqMan probe real-time polymerase chain reaction, was developed. The assay combined porcine-specific primers and TaqMan probe for the detection of a 109 bp fragment of porcine cytochrome b gene. Specificity test with 10 ng DNA of eleven different species yielded a threshold cycle (Ct) of 15.5 卤 0.20 for the pork and negative results for the others. Analysis of beef meatballs with spiked pork showed the assay can determine 100-0.01%contaminated pork with 102%PCR efficiency, high linear regression (r² = 0.994) and 鈮?#xA0;6%relative errors. Residuals analysis revealed a high precision in all determinations. Random analysis of commercial meatballs from pork, beef, chicken, mutton and goat, yielded a Ct between 15.89 卤 0.16 and 16.37 卤 0.22 from pork meatballs and negative results from the others, showing the suitability of the assay to determine pork in commercial meatballs with a high accuracy and precision.