Effects of metronidazole and tinidazole on NTPDase1 and ecto-5′-nucleotidase from intact cells of Trichomonas vaginalis
- 作者：Tasca ; Tiana ; Borges ; Fernanda Pires ; Bonan ; Carla Denise ; De Carli ; Geraldo Attilio ; Battastini ; Ana Maria Oliveira ; et. al.
- 关键词：NTPDase1 ; Ecto-5&prime ; -nucleotidase ; Metronidazole ; Tinidazole ; Trichomonas vaginalis
- 刊名：FEMS Microbiology Letters
- 出版年：2003
- 期刊代码：34_03781097
- 类别：imm
- 出版时间：September 26, 2003
- 卷：226
- 期：2
- 页码：379-384
- 文件大小：304 K
摘要
Here we report the effects of metronidazole and tinidazole on NTPDase1 and ecto-5′-nucleotidase from intact cells of Trichomonas vaginalis. Adenosine triphosphate (ATP) and adenosine diphosphate (ADP) hydrolysis was 5- to 7-fold higher for the fresh clinical strain, when compared with the ATCC (American Type Culture Collection) strain. ATP hydrolysis was activated in the presence of metronidazole in the ATCC strain, whilst it was inhibited 33%by 50 μM tinidazole in a fresh clinical isolate. The treatment of cells in the presence of metronidazole for 2 h inhibited ATP and ADP hydrolysis, whilst treatment with tinidazole inhibited ATP and ADP hydrolysis only in the fresh clinical isolate. The drugs did not change the ecto-5′-nucleotidase activity for both strains. Our results suggest that the modulation of extracellular ATP and ADP levels during treatment with these drugs could be a parasitic defence strategy as a survival mechanism in an adverse environment.