Impact of tillage system on arbuscular mycorrhiza fungal communities in the soil under Mediterranean conditions

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• 作者：Isabel Brito^a ; ^{ibrito@uevora.pt} ; Michael J. Goss^b ; Má ; rio de Carvalho^a ; Odile Chatagnier^c ; Diederik van Tuinen^c
• 关键词：Arbuscular mycorrhiza (AM) fungi ; Genetic diversity ; Soil tillage ; Large ribosomal subunit (LSU rDNA)
• 刊名：Soil & Tillage Research
• 出版年：2012
• 期刊代码：95_01671987
• 类别：abs
• 出版时间：May, 2012
• 卷：121
• 期：Complete
• 页码：63-67
• 文件大小：496 K

摘要

A more diverse arbuscular mycorrhiza (AM) fungal community should be more versatile and resilient to variation in environmental conditions over space and time. To evaluate the effect of no-till and conventional tillage systems, AM fungal diversity was assessed as part of a long term field experiment by sequencing of DNA, extracted from soil, that encoded the large ribosomal sub-unit and was obtained by nested-PCR. In comparison with no-till, conventional tillage decreased AM fungal diversity by 40%. Differences between treatments in the frequency of the operational taxonomic units (OTUs) present in soil, confirm that AM fungi are differently vulnerable to soil disturbance.