Identification and expression of alternative splice variants of the mouse Ppp1r3b gene in lung epithelial cells

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• 作者：Tomoaki Niimi ; Reiko Kurotani ; Shioko Kimura and Yasuo Kitagawa
• 关键词：TITF-1 ; Nkx2.1 ; Glycogen-targeting subunit ; Protein phosphatase-1 ; Lung development
• 刊名：Biochemical and Biophysical Research Communications
• 出版年：2006
• 期刊代码：159_0006291x
• 类别：bio
• 出版时间：20 October 2006
• 卷：349
• 期：2
• 页码：588-596
• 文件大小：1553 K

摘要

Hepatic glycogen-targeting subunit G_L of protein phosphatase-1 (PP1), which is encoded by the gene Ppp1r3b, is one of a family of proteins that target PP1 to glycogen and regulate its activity on enzymes of glycogen metabolism. G_L is primarily expressed in the liver where it promotes hepatic glycogen synthesis in response to insulin. Here, we show that G_L is highly expressed in embryonic mouse lungs. G_L utilizes two alternative promoters and 5′ non-coding exons, which produce at least three alternatively spliced transcripts that encode identical proteins. Expression of G_L in the lung is detectable as early as embryonic day (E) 12.5 and increases by E16.5, however, it declines before birth. In situ hybridization of mouse embryonic lung revealed that G_L is expressed in bronchial epithelial cells. Thus, the temporal and spatial expression of G_L in the lung suggests that it has a potential role in glycogen metabolism during lung development.