Use of a thiophene-based conducting polymer in microbial biosensing
- 作者：Dilek Odaci ; Senem Kiralp Kayahan ; Suna Timur ; Levent Toppare
- 关键词：Conducting polymer ; Electrochemical polymerization ; Microbial electrode ; Pseudomonas fluorescens ; Electrochemical biosensor
- 刊名：Electrochimica Acta
- 出版年：2008
- 期刊代码：30_00134686
- 类别：ch
- 出版时间：1 May 2008
- 卷：53
- 期：12
- 页码：4104-4108
- 文件大小：228 K
摘要
Immobilization of whole viable Pseudomonas fluorescens cells was achieved on a graphite electrode modified with a thiophene-based conducting polymer. Microbial electrodes were constructed by the entrapment of bacterial cells on conducting copolymer matrix using a dialysis membrane. The biosensor was characterized using glucose as the substrate. As well as analytical characterization, effects of electropolymerization time, pH and temperature on the sensor response were examined. Finally, operational stability was also tested.