Suppression of expression of muscle-associated proteins by PPARα in brown adipose tissue
- 作者：Yuhong Tong ; Atsushi Hara ; Makiko Komatsu ; Naoki Tanaka ; Yuji Kamijo ; Frank J. Gonzalez and Toshifumi Aoyama
- 关键词：Peroxisome proliferator-activated receptor α ; Brown adipose tissue ; Synchronous suppression ; Transcriptional regulation ; MALDI-TOF MS
- 刊名：Biochemical and Biophysical Research Communications
- 出版年：2005
- 期刊代码：159_0006291x
- 类别：bio
- 出版时间：2005
- 卷：336
- 期：1
- 页码：76-83
- 文件大小：438 K
摘要
Peroxisome proliferator-activated receptor α (PPARα) belongs to the steroid/nuclear receptor superfamily. Two-dimensional (2D) SDS–PAGE analysis of brown adipose tissue (BAT) unexpectedly revealed six spots that were present only in PPARα-null mice. Proteomic analysis indicated that these proteins were tropomyosin-1 α chain, tropomyosin β chain, myosin regulatory light chain 2, myosin light chain 3, and parvalbumin α. Analyses of mRNA have revealed that PPARα suppressed the genes encoding these proteins in a synchronous manner in adult wild-type mice. Histological and physiological analyses of BAT showed in adult wild-type mice, a marked suppression of BAT growth concurrent with a prominent decrease in lipolytic and thermogenesis activities. These results suggest that in adult mice, PPARα functions to suppress the expression of the proteins that may be involved in the architecture of BAT, and thus may function in keeping BAT in a quiescent state.