Effects of selected food factors with chemopreventive properties on combined lipopolysaccharide- and interferon-γ-induced IκB degradation in RAW264.7 macrophages

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• 作者：Murakami ; Akira ; Matsumoto ; Kazuhiko ; Koshimizu ; Koichi ; Ohigashi ; Hajime
• 关键词：Nuclear factor-kappaB ; Zerumbone ; Benzylisothiocyanate ; Genistein ; 1&prime ; -acetoxycahvicol acetate ; I&#954 ; B ; inhibitor kappaB ; NF-kB ; nuclear factor kappaB ; IKK ; I&#954 ; B kinase ; LPS ; lipopolysaccharide ; IFN ; interferon ; COX ; cyclooxygenase ; TNF ; tumor
• 刊名：Cancer Letters
• 出版年：2003
• 期刊代码：44_03043835
• 类别：med
• 出版时间：May 30, 2003
• 卷：195
• 期：1
• 页码：17-25
• 文件大小：370 K

摘要

Degradation of IkappaB (IκB) is a key step for nuclear factor-kappaB (NF-κB)-induced transcription of certain proinflammatory genes, including inducible nitric oxide synthase (iNOS) and cyclooxygenase (COX)-2. We selected seven chemopreventive agents and examined their effects on combined lipopolysaccharide- and interferon-γ-induced IκB degradation in RAW264.7 murine macrophages. IκB degradation was notably suppressed by 1′-acetoxychavicol acetate (ACA), zerumbone (ZER), and benzylisothiocyanate (BITC), however, not by auraptene (AUR), while the suppressive potencies of nobiletin (NOB), genistein (GEN), and resveratrol (RES) were low, but significant. These results suggest that ACA, ZER, and BITC suppress iNOS/COX-2 gene expression mainly by attenuating IκB degradation, while other chemopreventive agents use alternative pathway(s) to suppress the expression of proinflammatory genes.