Identification and characterization of zebrafish SULT1 ST9, SULT3 ST4, and SULT3 ST5

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• 作者：Yasir I. Mohammed^a ; Katsuhisa Kurogi^a ; Amani Al Shaban^a ; Zheng Xu^a ; Ming-Yih Liu^b ; Frederick E. Williams^a ; Yoichi Sakakibara^c ; Masahito Suiko^c ; Shakhawat Bhuiyan^d ; Ming-Cheh Liu^a ; ^{ming.liu@utoledo.edu}
• 关键词：SULT ; sulfotransferase ; PAPS ; 3&prime ; -phosphoadenosine-5&prime ; -phosphosulfate ; RT-PCR ; reverse transcription-polymerase chain reaction ; DHEA ; dehydroepiandrosterone
• 刊名：Aquatic Toxicology
• 出版年：2012
• 期刊代码：19_0166445x
• 类别：abs
• 出版时间：15 May, 2012
• 卷：112-113
• 期：Complete
• 页码：11-18
• 文件大小：614 K

摘要

By searching the GenBank database, we identified sequences encoding three new zebrafish cytosolic sulfotransferases (SULTs). These three new zebrafish SULTs, designated SULT1 ST9, SULT3 ST4, and SULT3 ST5, were cloned, expressed, purified, and characterized. SULT1 ST9 appeared to be mostly involved in the metabolism and detoxification of xenobiotics such as 尾-naphthol, 尾-naphthylamine, caffeic acid and gallic acid. SULT3 ST4 showed strong activity toward endogenous compounds such as dehydroepiandrosterone (DHEA), pregnenolone, and 17尾-estradiol. SULT3 ST5 showed weaker, but significant, activities toward endogenous compounds such as DHEA and corticosterone, as well as xenobiotics including mestranol, 尾-naphthylamine, 尾-naphthol, and butylated hydroxyl anisole (BHA). pH-dependency and kinetic constants of these three enzymes were determined with DHEA, 尾-naphthol, and 17尾-estradiol as substrates. Reverse transcription-polymerase chain reaction (RT-PCR) was performed to examine the expression of these three new zebrafish SULTs at different developmental stages during embryogenesis, through larval development, and on to maturity.