Evaluation of the PrimerDesign鈩?genesig real-time reverse transcription-polymerase chain reaction assay and the INFINITI庐 Respiratory Viral Panel Plus assay for the detection of human metapneumovirus in Kuwait

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• 作者：Mariam Al-Turab^a ; ^{mariamalturab@hsc.edu.kw} ; Wassim Chehadeh^a ; Fahd Al-Mulla^b ; Widad Al-Nakib^a
• 关键词：hMPV ; human metapneumovirus ; RVP-Plus ; Respiratory Viral Panel Plus ; RT-PCR ; reverse transcription PCR
• 刊名：Diagnostic Microbiology and Infectious Disease
• 出版年：2012
• 期刊代码：79_07328893
• 类别：med
• 出版时间：April, 2012
• 卷：72
• 期：4
• 页码：358-362
• 文件大小：197 K

摘要

Human metapneumovirus (hMPV) is a respiratory pathogen that was discovered in 2001 and is considered a major cause of both upper and lower respiratory tract infections. A sensitive, fast, and high-throughput diagnostic test is needed for the detection of hMPV that may assist in the clinical management as well as in the reduction of inappropriate therapy. Therefore, a comparison assessment was performed in this study between the PrimerDesign鈩?genesig real-time reverse transcription-polymerase chain reaction (RT-PCR) Assay and the INFINITI^庐 Respiratory Viral Panel Plus Assay (RVP-Plus) for the detection of hMPV infection in patients with respiratory tract infections. A total of 200 respiratory samples were collected from 185 hospitalized patients, during the winter season in Kuwait. Of 185 patients, 10 (5.4%) were positive for hMPV RNA by the in-house RT-PCR assay, while 7 (4%) were positive for hMPV RNA by the real-time RT-PCR assay and 9 (5%) were positive for hMPV RNA by the INFINITI^庐 RVP-Plus assay. The high incidence rate (60%) of hMPV infection was in January 2011. The sensitivity of the real-time RT-PCR and INFINITI^庐 RVP-Plus assays was 70%and 90%, respectively, with specificity of 100%for both assays. hMPV types A and B could be identified in this study; however, discordant genotyping results were found between the direct sequencing method and the INFINITI^庐 RVP-Plus assay in 33%of hMPV-positive patients.